Review



rabbit monoclonal primary antibodies against human p21  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal primary antibodies against human p21
    Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and <t>p21.</t> The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.
    Rabbit Monoclonal Primary Antibodies Against Human P21, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 3049 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+monoclonal+primary+antibodies+against+human+p21/p21+Waf1%2FCip1+Rabbit+mAb/pm33169800-100-25-100
    Average 97 stars, based on 3049 article reviews
    rabbit monoclonal primary antibodies against human p21 - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro."

    Article Title: MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro.

    Journal: Oncology reports

    doi: 10.3892/or.2020.7844

    Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and p21. The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.
    Figure Legend Snippet: Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and p21. The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.

    Techniques Used: Transfection, TUNEL Assay, Western Blot, Expressing, Flow Cytometry, Negative Control

    Related Articles

    Incubation:

    Article Title: MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro
    Article Snippet: Cells were harvested using RIPA lysis buffer, Total proteins (40 μg/lane) were separated via 12% SDS-PAGE and were transferred onto PVDF membranes (EMD Millipore) using a MiniGenie blotting system (Bio-Rad Laboratories, Inc.). .. The membranes were then blocked with TBS-Tween (TBST; 0.1% Tween-20) containing 1% skim milk powder at room temperature for 1 h, and then incubated with rabbit monoclonal primary antibodies against human p21 (1:1,000; cat. no. 2947S), cyclooxygenase-2 (Cox-2; 1:1,000; cat. no. 12282T), matrix metalloproteinase (MMP)2 (1:1,000; cat. no. 40994S), MMP9 (1:1,000; cat. no. 13667S), cyclin D1 (1:1,000; cat. no. 55506S), CDK2 (1:1,000; cat. no. 2546S), Bax (1:1,000; cat. no. 5023S), cleaved caspase-3 (1:1,000; cat. no. 9654S), cleaved caspase-9 (1:1,000; cat. no. 20750S), Bcl-2 (1:1,000; cat. no. 4223S), β-actin (1:1,000; cat. no. 4970T) and FEN1 (1:2,000; cat. no. 82354S) (all from Cell Signaling Technology, Inc.) at 4°C overnight. .. After washing with TBST, the membranes were incubated with goat anti-rabbit secondary antibodies (1:10,000; cat. no. 14708S; Cell Signaling Technology, Inc.) for 1 h at room temperature, followed by visualization with an enhanced chemiluminescence system (BeyoECL Plus; Beyotime Institute of Biotechnology).

    Article Title: MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro.
    Article Snippet: Cells were harvested using RIPA lysis buffer, Total proteins (40 μg/lane) were separated via 12% SdS-PAGE and were transferred onto PVdF membranes (EMd Millipore) using a MiniGenie blotting system (Bio-Rad Laboratories, Inc.). .. The membranes were then blocked with TBS-Tween (TBST; 0.1% Tween-20) containing 1% skim milk powder at room temperature for 1 h, and then incubated with rabbit monoclonal primary antibodies against human p21 (1:1,000; cat. no. 2947S), cyclooxygenase-2 (Cox-2; 1:1,000; cat. no. 12282T), matrix metalloproteinase (MMP)2 (1:1,000; cat. no. 40994S), MMP9 (1:1,000; cat. no. 13667S), cyclin d1 (1:1,000; cat. no. 55506S), CdK2 (1:1,000; cat. no. 2546S), Bax (1:1,000; cat. no. 5023S), cleaved caspase-3 (1:1,000; cat. no. 9654S), cleaved caspase-9 (1:1,000; cat. no. 20750S), Bcl-2 (1:1,000; cat. no. 4223S), β-actin (1:1,000; cat. no. 4970T) and FEN1 (1:2,000; cat. no. 82354S) (all from Cell Signaling Technology, Inc.) at 4 ̊C overnight. .. After washing with TBST, the membranes were incubated with goat anti-rabbit secondary antibodies (1:10,000; cat. no. 14708S; Cell Signaling Technology, Inc.) for 1 h at room temperature, followed by visualization with an enhanced chemiluminescence system (BeyoECL Plus; Beyotime Institute of Biotechnology).



    Similar Products

    rabbit monoclonal primary antibodies against human p21  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc rabbit monoclonal primary antibodies against human p21
    Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and <t>p21.</t> The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.
    Rabbit Monoclonal Primary Antibodies Against Human P21, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+monoclonal+primary+antibodies+against+human+p21/p21+Waf1%2FCip1+Rabbit+mAb/pm33169800-100-25-100
    Average 97 stars, based on 1 article reviews
    rabbit monoclonal primary antibodies against human p21 - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and p21. The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.

    Journal: Oncology reports

    Article Title: MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro.

    doi: 10.3892/or.2020.7844

    Figure Lengend Snippet: Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and p21. The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.

    Article Snippet: The membranes were then blocked with TBS-Tween (TBST; 0.1% Tween-20) containing 1% skim milk powder at room temperature for 1 h, and then incubated with rabbit monoclonal primary antibodies against human p21 (1:1,000; cat. no. 2947S), cyclooxygenase-2 (Cox-2; 1:1,000; cat. no. 12282T), matrix metalloproteinase (MMP)2 (1:1,000; cat. no. 40994S), MMP9 (1:1,000; cat. no. 13667S), cyclin d1 (1:1,000; cat. no. 55506S), CdK2 (1:1,000; cat. no. 2546S), Bax (1:1,000; cat. no. 5023S), cleaved caspase-3 (1:1,000; cat. no. 9654S), cleaved caspase-9 (1:1,000; cat. no. 20750S), Bcl-2 (1:1,000; cat. no. 4223S), β-actin (1:1,000; cat. no. 4970T) and FEN1 (1:2,000; cat. no. 82354S) (all from Cell Signaling Technology, Inc.) at 4 ̊C overnight.

    Techniques: Transfection, TUNEL Assay, Western Blot, Expressing, Flow Cytometry, Negative Control